From: Pleiotropic effects of RsmA and RsmE proteins in Pseudomonas fluorescens 2P24
Strains, plasmids or oligonucletoide | Relevant characteristics* | Reference or source |
---|---|---|
Strains | ||
Pseudomonas fluorescens | ||
 2P24 | Wild-type, Apr | [16] |
 PM206 | In-frame deletion of retS, Apr | [34] |
 WPM30 | In-frame deletion of phlD, Apr | This work |
 WPM12 | Double deletion of rsmA and rsmE, Apr | [15] |
 WPM31 | Triple deletion of rsmA, rsmE, and phlD, Apr | This work |
E. coli DH5α | supE44 lacU169 (ϕ80lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [35] |
Plasmids | ||
 p2P24Km | Sucrose-based counter-selectable plasmid, Kmr | [36] |
 p2P24Km-phlD | Plasmid p2P24Km carrying a deleted phlD gene, Kmr | This work |
 p970Km-phlFp | phlF-lacZ transcriptional fusion, Kmr | This work |
 p970Km-phlGp | phlG-lacZ transcriptional fusion, Kmr | This work |
 p415-phlG | pRK415 containing the phlG gene, Tetr | [22] |
 pHSG299 | Cloning vector, Kmr | TaKaRa |
Oligonucletoides | Sequence (5′-′3) a | Comment |
phlD-F1-EcoRI | AAGAATTCATGGCGATGGTGCGCCT | phlD null mutant construction |
phlD-R1–680 | GAATTTTCCGTCCGCCTGTATGGAACATGAAACCCGTGCACGATGTCACA | |
phlD-F2–700 | TGTGACATCGTGCACGGGTTTCATGTTCCATACAGGCCGGACGGAAAATTC | |
phlD-R2-SalI | AAGTCGACCAGGCTGGTGATCAATG | |
phlG-PFBamHI | TAGGATCCAGTTGCA CCAACCGAGC | phlG-lacZ transcriptional fusion |
phlG-PRBamHI | ATGGATCCGGCACGCTGATCTTCGAGC | |
phlF-PFBamHI | ACGGATCCAGATCTTAAGGGTTTCTAT | phlF-lacZ transcriptional fusion |
phlF-PRBamHI | GTGGATCCATAAGGATTGGTGCAG |