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Table 1 bacterial strains, plasmids, and oligonucletoides used in this study

From: Pleiotropic effects of RsmA and RsmE proteins in Pseudomonas fluorescens 2P24

Strains, plasmids or oligonucletoide

Relevant characteristics*

Reference or source

Strains

Pseudomonas fluorescens

 2P24

Wild-type, Apr

[16]

 PM206

In-frame deletion of retS, Apr

[34]

 WPM30

In-frame deletion of phlD, Apr

This work

 WPM12

Double deletion of rsmA and rsmE, Apr

[15]

 WPM31

Triple deletion of rsmA, rsmE, and phlD, Apr

This work

E. coli DH5α

supE44 lacU169 (Ï•80lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

[35]

Plasmids

 p2P24Km

Sucrose-based counter-selectable plasmid, Kmr

[36]

 p2P24Km-phlD

Plasmid p2P24Km carrying a deleted phlD gene, Kmr

This work

 p970Km-phlFp

phlF-lacZ transcriptional fusion, Kmr

This work

 p970Km-phlGp

phlG-lacZ transcriptional fusion, Kmr

This work

 p415-phlG

pRK415 containing the phlG gene, Tetr

[22]

 pHSG299

Cloning vector, Kmr

TaKaRa

Oligonucletoides

Sequence (5′-′3) a

Comment

phlD-F1-EcoRI

AAGAATTCATGGCGATGGTGCGCCT

phlD null mutant construction

phlD-R1–680

GAATTTTCCGTCCGCCTGTATGGAACATGAAACCCGTGCACGATGTCACA

phlD-F2–700

TGTGACATCGTGCACGGGTTTCATGTTCCATACAGGCCGGACGGAAAATTC

phlD-R2-SalI

AAGTCGACCAGGCTGGTGATCAATG

phlG-PFBamHI

TAGGATCCAGTTGCA CCAACCGAGC

phlG-lacZ transcriptional fusion

phlG-PRBamHI

ATGGATCCGGCACGCTGATCTTCGAGC

phlF-PFBamHI

ACGGATCCAGATCTTAAGGGTTTCTAT

phlF-lacZ transcriptional fusion

phlF-PRBamHI

GTGGATCCATAAGGATTGGTGCAG

  1. *Ap, ampicillin; Km, kanamycin; Tet, tetracycline
  2. aRestriction site inserted in the primer for the cloning strategy are underlined