BMC Microbiology

Table 1 Reproducibility of the assays on diluted plasmid DNA containing cloned target sequences

From: Novel multiplex real-time PCR assays reveal a high prevalence of diarrhoeagenic Escherichia coli pathotypes in healthy and diarrhoeal children in the south of Vietnam

Target sequence	Co-efficient of variance (%)	Target concentration
Target sequence	Co-efficient of variance (%)	5 × 10⁷	5 × 10⁶	5 × 10⁵	5 × 10⁴	5 × 10³	5 × 10²	5 × 10¹	5 × 10⁰
uidA	Intra-assay variation ^a	0.13	0.09	0.14	0.26	0.34	0.12	0.82	0.52
uidA	Inter-assay variation ^b	0.18	0.18	0.21	0.73	0.66	0.74	0.66	0.93
eltB	Intra-assay variation	0.03	0.04	0.06	0.10	0.35	0.16	0.27	0.35
eltB	Inter-assay variation	0.07	0.11	0.05	0.23	0.41	0.50	0.38	0.67
estA	Intra-assay variation	0.01	0.05	0.03	0.25	0.19	0.16	0.15	0.06
estA	Inter-assay variation	0.15	0.03	0.04	0.05	0.52	0.36	0.12	0.76
aggR	Intra-assay variation	0.11	0.08	0.08	0.15	0.20	0.37	0.29	–
aggR	Inter-assay variation	0.06	0.11	0.33	0.03	0.29	0.23	1.05	–
ipaH	Intra-assay variation	0.19	0.17	0.20	0.12	0.12	0.60	0.15	0.11
ipaH	Inter-assay variation	0.23	0.28	0.20	0.07	0.42	0.20	0.52	1.42
eae	Intra-assay variation	0.07	0.24	0.29	0.06	0.26	0.76	0.20	0.48
eae	Inter-assay variation	0.38	0.16	0.06	0.46	0.82	0.64	0.63	0.88
bfpA	Intra-assay variation	0.03	0.09	0.11	0.17	0.50	0.90	0.63	0.90
bfpA	Inter-assay variation	0.01	0.11	0.05	0.06	0.18	0.38	0.75	1.23
rfbE	Intra-assay variation	0.03	0.11	0.09	0.42	0.20	0.70	0.98	0.30
rfbE	Inter-assay variation	0.02	0.04	0.10	0.23	0.60	1.49	1.29	1.08
stx1	Intra-assay variation	0.13	0.13	0.17	0.21	0.50	0.74	0.97	0.60
stx1	Inter-assay variation	0.22	0.50	0.71	0.98	1.19	1.57	1.95	0.55
stx2	Intra-assay variation	0.11	0.14	0.18	0.17	0.25	1.54	0.81	0.24
stx2	Inter-assay variation	0.12	0.22	0.43	0.47	0.87	1.54	2.12	1.97

^a Intra-assay variation was calculated by measuring the co-efficient of variance of the Ct value on three concurrently run assays
^b Inter-assay variation was calculated by comparing variation in Ct value on three independently run assays

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