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Table 7 Overview of the different pure culture experiments conducted

From: Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

 

Bacteriuma (inoculum size, v/v)

Ratio of B. fibrisolvens (%)/C. acnes (%) in the inoculumb

Growth mediumc

Fatty acid substrated

Incubation period (h)

Exp. 1

28 strains individually (Table 1) (5%)

NA

Control

18:3n-3

24

18:2n-6

24

trans-11 18:1

24

Exp. 2

28 strains individually (Table 1) (5%)

NA

Lactate

18:2n-6

24

Exp. 3

B. fibrisolvens (5%)

100/0

Control

18:2n-6

0, 2, 4, 8, and 24

Low pH

18:2n-6

0, 2, 4, 8, and 24

DHA

18:2n-6

0, 2, 4, 8, and 24

C. acnes (5%)

0/100

Control

18:2n-6

0, 2, 4, 8, and 24

Low pH

18:2n-6

0, 2, 4, 8, and 24

DHA

18:2n-6

0, 2, 4, 8, and 24

Exp. 4

B. fibrisolvens (10%)

100/0

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

B. fibrisolvens (5%) and C. acnes (5%)

50/50

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

B. fibrisolvens (1%) and C. acnes (9%)

10/90

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

B. fibrisolvens (0.2%) and C. acnes (9.8%)

2/98

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

B. fibrisolvens (0.04%) and C. acnes (9.96%)

0.4/99.6

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

C. acnes (10%)

0/100

Control

18:2n-6

0, 4, 8, and 24

Low pH

18:2n-6

24

DHA

18:2n-6

24

  1. a Fresh cultures, grown in modified control medium (30% rumen fluid instead of 20%, v/v) for 12 to 36 h, depending on the growth rate of the strain (OD600 = 1.26 ± 0.41; mean ± SD), were used as inoculum
  2. b NA, not applicable
  3. c Lactate, control medium supplemented with 200 mM Na-lactate; low pH, control medium with pH adjusted to 5.5; DHA (docosahexaenoic acid), control medium containing 40 μg/mL of 22:6n-3
  4. d The initial amount of fatty acid was 40 μg/mL