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Table 1 Metabolism of 18:3n-3 and 18:2n-6a by different bacterial strains during 24 h of incubation under control growth conditions (Exp. 1). Blank cells related to 18:3n-3 and 18:2n-6 indicate no metabolism takes place by these strains

From: Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

Strain

Total VFA formed (μmol/tube; mean ± SD)b

VFA productsc

18:3n-3

18:2n-6

% metabolized (mean ± SD)d

Products formed (% of total intermediates formed and remaining 18:3n-3)e,f

% metabolized (mean ± SD)d

Products formed (% of total intermediates and remaining 18:2n-6)e,f

Acidaminococcus fermentans VR4

54 ± 9.5

A, B

    

Acidaminococcus intestini ADV 255.99

55 ± 13.0

A, B

    

Bifidobacterium adolescentis RU 424

251 ± 5.4

A

79.8 ± 13.94

10-OH ∆12,15–18:2 (45%)

76.8 ± 6.86

10-OH ∆12–18:1 (62%)

Bifidobacterium pseudolongum RU224

210 ± 18.5

A

26.7 ± 14.27

10-OH ∆12,15–18:2 (13%)

23.1 ± 8.56

10-OH ∆12–18:1 (15%)

Butyrivibrio fibrisolvens D1

102 ± 45.9

B, A

99.3 ± 0.43

c9,t11,c15 CLnA (48%)

98.0 ± 0.40

t11 18:1 (89%)

t11,c15 18:2 (43%)

t11 C18:1 (6%)

Butyrivibrio proteoclasticus P18

169 ± 12.7

B, A

98.9 ± 0.03

c9/t13/t14 18:1g (42%)

98.4 ± 0.48

18:0 (76%)

t15/c11 18:1g (21%)

t11 18:1 (17%)

c15 18:1 (17%)

18:0 (6%)

t11, c15 18:2 (6%)

Lactobacillus ruminis RF1

30 ± 17.2

A, P

    

Lactobacillus ruminis RF2

14 ± 11.9

A, P

    

Cutibacterium acnes DSM 1897

99 ± 43.7

P, A

86.5 ± 15.83

∆11,13,15–18:3 (50%)

88.4 ± 7.16

t10,c12 CLA (75%)

t10,c12,c15 CLnA (5%)

10-OH 12–18:1 (7%)

Ruminococcus albus 7

24 ± 8.6

A

    

Streptococcus equinus Pearl 11

18 ± 5.2

A

21.6 ± 10.58

13-OH ∆9,15–18:2 (6%)

84.0 ± 2.95

13-OH ∆9–18:1 (69%)

Streptococcus gallolyticus DSM 16831

16 ± 5.1

A

96.3 ± 1.49

13-OH ∆9,15–18:2 (86%)

89.8 ± 2.99

13-OH ∆9–18:1 (47%)

∆9,14–18:2 (32%)

Megasphaera elsdenii B159

147 ± 5.6

B

    

Megasphaera elsdenii T81

126 ± 6.1

B

    

Megasphaera elsdenii LC1

121 ± 11.2

B, A

    

Megasphaera elsdenii 2602A

191 ± 21.2

B, P

32.7 ± 8.92

13-OH ∆9,15–18:2 (19%)

81.8 ± 3.71

13-OH ∆9–18:1 (63%)

∆9,14–18:2 (5%)

Megasphaera elsdenii 3016B

138 ± 10.5

B

    

Megasphaera elsdenii 3218A

134 ± 7.9

B

    

Megasphaera elsdenii 3436A

117 ± 4.3

B

    

Megasphaera elsdenii 4251

125 ± 6.8

B

    

Megasphaera elsdenii 4257

124 ± 5.3

B

    

Megasphaera elsdenii 4296

120 ± 5.5

B

    

Megasphaera elsdenii 4400

58 ± 25.8

A, P

    

Megasphaera elsdenii 5045

128 ± 6.6

B

    

Megasphaera elsdenii 5052B

63 ± 9.9

A

60.9 ± 11.21

10-OH ∆12,15–18:2 (34%)

88.0 ± 2.20

13-OH ∆9–18:1 (42%)

10-OH ∆12–18:1 (24%)

Megasphaera elsdenii 5596

127 ± 3.1

B

    

Selenomonas ruminantium GA-192

83 ± 12.0

P, A

    

Selenomonas ruminantium PC 18

241 ± 31.8

P, A

    
  1. a The initial amount of fatty acid was 40 μg/mL
  2. b Measured fermentation products were acetate, propionate, isobutyrate, butyrate, isovalerate, valerate and caproate
  3. c Main VFA product: A, acetate; B, butyrate; P, propionate; in decreasing order of importance. Lactate concentration was not measured
  4. d % metabolized, proportion of the initial 18:3n-3 or 18:2n-6 which was converted after 24 h of incubation
  5. e Only the intermediates representing ≥5% are presented as its proportion of the sum of total intermediates and remaining initial 18:3n-3 or 18:2n-6 after 24 h of incubation
  6. fc, cis; t, trans; CLA, conjugated linoleic acid; CLnA, conjugated linolenic acid. For each of the formed intermediates, the proportion of the respective intermediate on the sum of total produced intermediates and remainder of the initial product (i.e. 18:3n-3 or 18:2n-6) after 24 h was calculated
  7. g The different isomers could not be separated from each other with the used technique
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BMC Microbiology

ISSN: 1471-2180

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