Skip to main content

Table 1 The MICs determination and genetic mutations of tetracycline target sites in Erava-induced resistant S. aureus isolates

From: Eravacycline susceptibility was impacted by genetic mutation of 30S ribosome subunits, and branched-chain amino acid transport system II carrier protein, Na/Pi cotransporter family protein in Staphylococcus aureus

Strains MICs values (mg/L) Mutation of 30S ribosome subunits
Omada Tige Erava RR1 RR2 RR3 RR4 RR5 S10
CHS221(parental strain) 0.5 0.5 0.25 W W W W W W
CHS221-E1a 16 32 16 T170G G77A;
A1124G
C810T
G848C
G185A;
G1036A
W W
CHS221-E2a 32 32 16 T170G G77A;
A1124G
G742A;
C810T
G185A;
G1036A
W W
CHS165(parental strain) 0.5 0.5 0.25 W W W W W W
CHS165-E1a 32 32 8 T170G;
A972T
A1124G C810T G185A;
G1036A
W Tyr87His
CHS165-E2a 32 64 16 T170G
G848C
A1124G C810T G185A;
G1036A
G848A Let47Let;
Tyr87His
149(parental strain) 0.5 0.25 0.06 W W W W W W
149-E1a 16 8 8 T170G; G77A;
A1124G
C810T G185A;
G1036A
W Let47His;
149-E2a 32 32 32 T170G G77A;
AG847-848TA; A1124G
C810T G185A;
G1036A
W Ile46Ile
CHS759(parental strain) 0.5 0.25 0.25 W W W W W W
CHS759-E1a 16 8 8 T170G A1124G C810T G1036A W W
CHS759-E2a 16 32 16 T170G G77A;
A1124G
C810T G185A;
G1036A
T1257C W
CHS810(parental strain) 0.5 0.25 0.25 W W W W W W
CHS810-E1a 16 32 8 T170G A1124G C810T G1036A W Tyr87His
CHS810-E2a 16 32 8 T170G;
G848C
G77A;
A1124G
C810T G1036A W Tyr87His
CHS820(parental strain) 0.5 1 0.125 W W W W W W
CHS820-E1a 16 64 16 T170G G77A;
A1124G
C810T G185A;
G1036A
C1254T Let47His
CHS820-E2a 16 64 16 T170G G77A;
A1124G
C810T G185A;
G1036A
C1254T;
G1261C
Let47His
N315(parental strain) 0.5 0.5 0.25 W W W W W W
N315-E1a 8 8 8 T170G;
G1041A
A1124G C810T;
G848C
G185A T846A Tyr87His
N315-E2a 8 8 8 T170G;
G1041A
A1124G C810T G185A W Tyr87His
MS4(parental strain) 0.5 0.25 0.25 W W W W W W
MS4-E1a 16 32 16 T170G A1124G C810T G1036A W Let47His
  1. a: Erava-induced resistant isolates, respectively; RR1–5: five separate copies of the 16S rRNA gene; W, wild-type (no mutation)