Eravacycline susceptibility was impacted by genetic mutation of 30S ribosome subunits, and branched-chain amino acid transport system II carrier protein, Na/Pi cotransporter family protein in Staphylococcus aureus

Table 1 The MICs determination and genetic mutations of tetracycline target sites in Erava-induced resistant S. aureus isolates

Strains	MICs values (mg/L)			Mutation of 30S ribosome subunits
Strains	Omada	Tige	Erava	RR1	RR2	RR3	RR4	RR5	S10
CHS221(parental strain)	0.5	0.5	0.25	W	W	W	W	W	W
CHS221-E1^a	16	32	16	T170G	G77A; A1124G	C810T G848C	G185A; G1036A	W	W
CHS221-E2^a	32	32	16	T170G	G77A; A1124G	G742A; C810T	G185A; G1036A	W	W
CHS165(parental strain)	0.5	0.5	0.25	W	W	W	W	W	W
CHS165-E1^a	32	32	8	T170G; A972T	A1124G	C810T	G185A; G1036A	W	Tyr87His
CHS165-E2^a	32	64	16	T170G G848C	A1124G	C810T	G185A; G1036A	G848A	Let47Let; Tyr87His
149(parental strain)	0.5	0.25	0.06	W	W	W	W	W	W
149-E1^a	16	8	8	T170G;	G77A; A1124G	C810T	G185A; G1036A	W	Let47His;
149-E2^a	32	32	32	T170G	G77A; AG847-848TA; A1124G	C810T	G185A; G1036A	W	Ile46Ile
CHS759(parental strain)	0.5	0.25	0.25	W	W	W	W	W	W
CHS759-E1^a	16	8	8	T170G	A1124G	C810T	G1036A	W	W
CHS759-E2^a	16	32	16	T170G	G77A; A1124G	C810T	G185A; G1036A	T1257C	W
CHS810(parental strain)	0.5	0.25	0.25	W	W	W	W	W	W
CHS810-E1^a	16	32	8	T170G	A1124G	C810T	G1036A	W	Tyr87His
CHS810-E2^a	16	32	8	T170G; G848C	G77A; A1124G	C810T	G1036A	W	Tyr87His
CHS820(parental strain)	0.5	1	0.125	W	W	W	W	W	W
CHS820-E1^a	16	64	16	T170G	G77A; A1124G	C810T	G185A; G1036A	C1254T	Let47His
CHS820-E2^a	16	64	16	T170G	G77A; A1124G	C810T	G185A; G1036A	C1254T; G1261C	Let47His
N315(parental strain)	0.5	0.5	0.25	W	W	W	W	W	W
N315-E1^a	8	8	8	T170G; G1041A	A1124G	C810T; G848C	G185A	T846A	Tyr87His
N315-E2^a	8	8	8	T170G; G1041A	A1124G	C810T	G185A	W	Tyr87His
MS4(parental strain)	0.5	0.25	0.25	W	W	W	W	W	W
MS4-E1^a	16	32	16	T170G	A1124G	C810T	G1036A	W	Let47His

^a: Erava-induced resistant isolates, respectively; RR1–5: five separate copies of the 16S rRNA gene; W, wild-type (no mutation)

ISSN: 1471-2180