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Fig. 2 | BMC Microbiology

Fig. 2

From: Eravacycline susceptibility was impacted by genetic mutation of 30S ribosome subunits, and branched-chain amino acid transport system II carrier protein, Na/Pi cotransporter family protein in Staphylococcus aureus

Fig. 2

No significant elevation of the relative transcriptional expression level of the five candidate genes, including USA300HOU_RS00705, USA300HOU_RS13205, USA300HOU_RS13945, USA300HOU_RS10505, USA300HOU_RS00660 and tetL in heteroresistance or Erava-resistant clones compared with that of the parental. The origin represented the parental isolates including CHS237, CHS632, CHS62, CHS239, N315 and MS4; Resistance represented the corresponding heteroresistance isolates CHS237-E1, CHS632-E1, CHS62-E1, CHS239-E1, which was reported previously [6], and two Erava-resistant isolates shown in Table 1, including N315-E1 and MS4-E1. Relative expression of USA300HOU_RS00705 (a), USA300HOU_RS13205 (b), USA300HOU_RS13945 (c), USA300HOU_RS10505 (d), USA300HOU_RS00660(e), tet(L) (f), were demonstrated by RT-qPCR analysis. The housekeeping gene gyrB was used as the endogenous reference gene. The original (parental) strain was used as the reference strain (expression = 1.0). All RT-qPCR were carried out in triplicate. *p < 0.5; **p < 0.01 (Student’s t-tests)

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BMC Microbiology

ISSN: 1471-2180

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