Fig. 4

Cell length and morphology of strain 724,644 cultured in the presence and absence of β-lactam antibiotics. The dot plot (a) represents the distribution of cell lengths (n = 95–100) measured (μm) using the thinned length object feature. Cells in media without antibiotics were measured after 2 h and 4 h and cell exposed to amoxicillin-clavulanic acid (AMC), ceftazidime (CAZ) and imipenem (IPM) were measured after 4 h. Median cell lengths were calculated (black lines). CLSI breakpoint for susceptibility (green underline) and MICs (blue underline). Optical screen images taken over time (b) in the presence of the MIC value of AMC