Fig. 3

Organization and molecular analysis of the biotin operons of Wolbachia. a Organization of the biotin operon. The operons among the Wolbachia and four outgroups were identified and then aligned using MAFFT [46]. b Phylogeny of the biotin operon (concatenation of contig containing bioA and bioD with the contig containing bioH, bioF and bioB). The length of datasets is 4603 bp. The topology was inferred using Maximum Likelihood (ML) inference using IQ-TREE [39]. The Best-fit model calculated using ModelFinder according to AIC index was TVM + I + G4. Nodes are associated with Bootstrap values based on 1000 replicates. The Wolbachia supergroups are indicated by the color: purple for supergroup F; dark blue for supergroup A; orange for supergroup A and green for supergroup S