| 18SV4BP | UNonMet | 18SV1V2EX |
---|---|---|---|
Advantages | BP specifically targets host sequences | No host sequences are expected to be amplified | No host sequences were amplified |
Protist diversity is expected to be well represented | High nucleotide polymorphism compared to 18SV4BP | ||
Disadvantages | Host sequences were not completely removed | Excluding primers might not only exclude metazoans, but also other unexpected taxa | |
Expected amplicon size is too large for current Illumina MiSeq technology | Â | ||
Low nucleotide polymorphism compared to 18SV1V2EX |