Table 6 Primer sequences and PCR conditions used to detect the hcp1, hcp3, lpfA, gipA and fimH genes
Gene | Primer sequencing (5’- 3′) | PCR conditions | Product (pb) | Reference |
|---|---|---|---|---|
hcp1 | F: AAACACCACTGGAGTACCTG R: TGGTACTTAGCAAGAAAGAGC | 95 °C 3 min., 35 cycles (95 °C 45 s., 55 °C 30 s, e 72 °C 45 s.) e 68 °C 8 min. | 999 | This study |
hcp3 | F: CGAAATCAGTCTTGTTCCGC R: GGGTTTCCTTATCGTGTTCT | 95 °C 3 min., 35 cycles (95 °C 45 s., 55 °C 30 s, e 72 °C 45 s.) e 68 °C 8 min. | 745 | This study |
lpfA | F: GGCCTTCTTTCAGACGGTA R: CTGGAAAACTGCGATATCTCC | 95 °C 3 min., 35 cycles (95 °C 45 s., 57 °C 30 s, e 72 °C 45 s.) e 68 °C 8 min. | 199 | [45] |
gipA | F: GTCGTTGCGCCACCAACAA R: ACGGCGCAGATGGTAATTCT | 94 °C 5 min., 35 cycles (94 °C 1 m., 55 °C 1 m, e 72 °C 30 s.) e 72 °C 10 min. | 200 | [46] |
fimH | F: ATTCCTCACAATCAGCGCAC R: CGCGTCTTATCTGGCCTACA | 95 °C 3 min., 35 cycles (95 °C 45 s., 57 °C 30 s, e 72 °C 45 s.) e 68 °C 8 min. | 1125 | This study |