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Fig. 2 | BMC Microbiology

Fig. 2

From: Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA

Fig. 2

Phenotypes of the wide type S. Typhimurium and ECA-defective mutant strain. a Growth curves. Overnight cultures were diluted to an optical density of 0.025 and incubated at 37 °C with shaking at 180 rpm; the OD600 was measured at the appropriate time. b Bile sensitivity assay. Three-microliters of a 10-fold dilution of the overnight cultures of the wide type χ3761 and mutant χ12357 strains were deposited and incubated for 12 h at 37 °C in LB plates with or without 1% DOC. c. LPS profiles. LPS from χ3761 and the mutant χ12357 were subjected to 12% SDS-PAGE and then silver stained

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