Fig. 2From: Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspAPhenotypes of the wide type S. Typhimurium and ECA-defective mutant strain. a Growth curves. Overnight cultures were diluted to an optical density of 0.025 and incubated at 37 °C with shaking at 180 rpm; the OD600 was measured at the appropriate time. b Bile sensitivity assay. Three-microliters of a 10-fold dilution of the overnight cultures of the wide type χ3761 and mutant χ12357 strains were deposited and incubated for 12 h at 37 °C in LB plates with or without 1% DOC. c. LPS profiles. LPS from χ3761 and the mutant χ12357 were subjected to 12% SDS-PAGE and then silver stainedBack to article page