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Fig. 1 | BMC Microbiology

Fig. 1

From: Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA

Fig. 1

ECA operon deletion in S. Typhimurium χ3761 a Map of the ECA operon. b Schematic strategy for deletion of the ECA operon by allelic exchange using suicide vectors; the whole ECA operon from wecA to wecG was deleted. c ECA operon deficiency mutation identification by PCR amplification. Primer pairs for Doperon-1F/Doperon-2R were used to amplify the ECA operon using the ECA-deficient S. Typhimurium mutant χ12357 (lane 1) and the wide type χ3761 (lane 2) as the PCR template. A PCR product of approximately 800 bp was observed for χ12357, but no bands were observed for wild type S. Typhimurium χ3761. d Detection of ECA using ECA-specific mAb898 antibody by western blotting. The wide type S. Typhimurium χ3761 could produce ECA, but the ECA-deficient Salmonella strain χ12357 lost the ability to produce ECA

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