Effects of CO2 limitation on the metabolism of Pseudoclostridium thermosuccinogenes

BMC Microbiology

Table 2 Comparison with other studies. Studies showing the effects of CO₂ limitation on succinic acid producers grown on glucose, in terms of growth and product yields. SA: succinate, AA: acetate, FA: formate, LA: lactate, EtOH: ethanol, PA: pyruvate. Biomass and product yields are given in mol/mol glucose. Growth rate in h⁻¹

Organism	SA	AA	FA	LA (PA)	EtOH	Biomass	Growth rate	CO₂ provided	Ref.
Anaerobiospirillum succiniciproducens^a	1.21	0.67	0.12	0.00	0.00	1.13	?	0.98 mol MgCO₃/mol glucose	[33]
Anaerobiospirillum succiniciproducens^a	0.43	0.16	0.12	0.87	0.09	0.44	?	0.065 mol MgCO₃/mol glucose	[33]
Actinobacillus succinogenes	0.69	0.84	0.88	?	0.18	1.07	?	1.00 mol MgCO₃/mol glucose	[28]
Actinobacillus succinogenes	0.19	0.81	1.20	?	0.71	1.08	?	0.10 mol MgCO₃/mol glucose	[28]
Mannheimia succiniciproducens^b	0.70	?	?	?	?	0.18 g/g	1.12	100% CO₂ at 0.25 vvm (23 mM)	[34]
Mannheimia succiniciproducens^b	0.60	?	?	?	?	0.13 g/g	0.78	37% CO₂ at 0.25 vvm (8.7 mM)	[34]
Escherichia coli AFP111^c	1.10	0.12	?	(0.20)	0.12	?	?	50% CO₂ at 0.42 vvm	[35]
Escherichia coli AFP111^c	0.79	0.09	?	(0.39)	0.08	?	?	3% CO₂ at 0.42 vvm	[35]
Escherichia coli BA207^d	1.46	0.48	?	(0.04)	?	1.72 g/l	?	100% CO₂ at 0.13 vvm	[36]
Escherichia coli BA207^d	1.07	0.27	?	(0.12)	?	0.42 g/l	?	0% CO₂ at 0.13 vvm	[36]
Corynebacterium glutamicum^e	0.87	0.28	?	0.42	?	–	0	100% CO₂	[37]
Corynebacterium glutamicum^e	0.29	0.13	?	1.22	?	–	0	0% CO₂	[37]
Pseudoclostridium thermosuccinogenes	0.64	0.69	0.53	0.29	0.05	?	0.33	20% CO₂ at 0.033 vvm	This study
Pseudoclostridium thermosuccinogenes	0.47	0.56	0.17	0.35	0.23	?	0.20	1% CO₂ at 0.033 vvm	This study

^aGrowth rate decreased by almost 50% during CO₂ limitation
^bM. succiniproducens shows severely suppressed growth at CO₂ concentrations below 8.7 mM
^cEscherichia coli AFP111 has mutations in pfl, ldhA, ptsG. During 0% CO₂ succinic acid yield dropped to 0.06
^dEscherichia coli BA207: E. coli K12, ΔpflB, ΔldhA, Δppc, pTrc-pncB-pyc
^eNon-growing high-density cell suspensions

ISSN: 1471-2180