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Table 1 Changes in cofactor fluxes during CO2 limitation. Moles of different cofactors or pathway intermediates generated per mole of glucose consumed, calculated using the different product yields as shown in Fig. 4 and stoichiometry of the pathways from phosphoenolpyruvate to the different fermentation products (i.e. the NADH/ATP-equivalents consumed/produced in glycolysis are not considered), as depicted in Fig. 1. Calculations based on the assumption that there is no difference in transhydrogenation by the malate shunt between 1 and 20% CO2, and that 1 ATP-equivalent is formed in the conversion from phosphoenolpyruvate to pyruvate (Additional file 2)

From: Effects of CO2 limitation on the metabolism of Pseudoclostridium thermosuccinogenes

 

1% CO2

20% CO2

Difference

Acetyl-CoA

0.79

0.73

0.05

Fdreduced (PFOR only)

0.62

0.20

0.42

Fdreduced

1.09

0.84

0.26

NADH

−1.99

−2.25

0.25

NADPH

−0.23

−0.05

−0.18

Total redox cofactors

−1.13

−1.45

0.33

ATP (acetate kinase only)

0.56

0.68

−0.13

ATP-equivalents

2.16

2.34

−0.18