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Fig. 5 | BMC Microbiology

Fig. 5

From: Effects of CO2 limitation on the metabolism of Pseudoclostridium thermosuccinogenes

Fig. 5

Selected results from differential expression analysis. Positive fold change represents an increase of mRNA coverage during CO2 limitation (i.e. 1% versus 20% CO2). In the volcano plots, green data points depict genes with a statistically significant increase (i.e. q-value < 0.05) during CO2 limitation and red data points those with a statistically significant decrease in coverage during CO2 limitation. Yellow data points correspond to the genes presented in the accompanying bar graph. In the bar graphs, the wide bars display the log2(fold change) and the narrow (yellow) bars display the mean coverage in FPKM during 1% CO2. a Results for potential alcohol dehydrogenases (including AdhE). b Results for two annotated pyruvate:ferredoxin oxidoreductases (PFOR), two annotated pyruvate formate lyases (PFL), and three (potential) PFL-activating enzymes. c Results for the ‘succinate operon’, harbouring fumarate hydratase (CDQ83_RS03365), an electron bifurcating NADH dehydrogenase/heterodisulfide reductase complex (CDQ83_RS03360–30), fumarate reductase (CDQ83_RS03325–20), and a hypothetical protein (CDQ83_RS03315; potentially a succinic acid transporter). d Results for the energy-converting [NiFe] hydrogenase with hydrogenase maturation factors (Ech; CDQ83_RS14500–45); the ion-translocating reduced ferredoxin: NAD+ oxidoreductase (Rnf; CDQ83_RS14555–75); HydEFG [FeFe] hydrogenase maturation factors; electron bifurcating ferredoxin: NADP+ reductase (Nfn; CDQ83_RS10790–95); HypCDE [NiFe] hydrogenase maturation factors (CDQ83_RS06905–15); and three potential hydrogenases (CDQ83_RS10810, CDQ83_RS11525–45, and CDQ83_RS16105)

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