Skip to main content

Table 2 Primers and conditions used in RT-PCR for cytokine gene expression analysis

From: Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria

Target gene

Primer sequence 5′-3′

Size (bp)

Annealing temperatures (°C)

References

TNF-α

F: GAAACCTGGGATTCAGGAATG

R: GTCTCAAGGAAGTCTGGAAAC

246

55

[56]

IL-8

F: GCAGCTCTGTGTGAAGGTGCAG

R: GCATCTGGCAACCCTACAACAG

365

55

[58]

GAPDH

F: ATCACCATCTTCCAGGAG

R: ATCGACTGTGGTCATGAG

318

55

[59]