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Fig. 1 | BMC Microbiology

Fig. 1

From: Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria

Fig. 1

Confocal laser scanning microscopy 3-dimensional (3D) images of monospecies and polymicrobial biofilms of bacteria and C. albicans. A. actinomycetemcomitans (Aa), S. mutans (Sm), S. gordonii (Sg) and C. albicans (Ca) were cultured as monospecies and polymicrobial (mix) in brucella broth in the wells of Millicell® EZ slides (Millipore) in aerobiosis in 5% CO2 at 37 °C for 24 and 48 h and stained with Syto9®. Images were acquired on Carl-Zeiss LSM 700 at × 630 magnification and 3D view reconstructed using the software ZEN 2012

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