Fig. 1

Specificity and sensitivity of PCR detection of motile lactobacilli with Lag/Lru primers (top) and universal primers (bottom). (a) PCR amplification with genomic DNA from eleven strains of motile lactobacilli and a non-motile Lactobacillus and an E. coli. The strains are listed in Table 2. (b) PCR amplification with DNA extracted from feces of a chicken (motile lactobacilli-positive) or a Siberian tiger (motile lactobacilli-negative). (c) PCR amplification with DNA extracted from murine fecal pellets supplemented with different concentrations of L. agilis BKN88 cells (1 × 10¹ to 10⁸ CFU/g feces)