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Table 4 Oligonucleotide primer pairs and protocol used for optimized species-specific real-time qPCR-based assays

From: Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri

SpeciesTarget GeneSequence
5ˊ-3ˊ
Anealing Temp (°C)Product Size (bp)Melting Peak (°C)Reference
A. faecisrpoBAfae-F: GCT CCA GGA AGT ACA AAA GTA G
Afae-R: AGG CTA GCA GCT ACT CCC
58.015279Khan et al. 2017 [15]
A. lanthierigyrAAlan-F: CTT GGT GAA TTG CTT GAT GCA A
Alan-R: CCA TTA AAT CAC TAG CTT CTG CT
55.07274This study