Fig. 3

Standard curves developed from DNA extracted from 10-fold serially diluted spiked cells of A. faecis (Panel A) (ranging from 10² to 10⁸ g^− 1) and A. lanthieri (Panel B) (ranging from 10² to 10⁷ g^{- 1}) in cow manure using species-specific qPCR assays. The standard curves obtained from amplification plot by correlation of the Quantification Cycle (Cq) cycle values and number of cells g^− 1 of feces. Each point is representing the result of duplicate amplification of each dilution where the correlation coefficients R² and the slopes of the regression curve are shown