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Table 3 Primers and probes used for H. pylori rt-PCR assay

From: Assessment of real-time PCR for Helicobacter pylori DNA detection in stool with co-infection of intestinal parasites: a comparative study of DNA extraction methods

Primer/probe nameAccession numberPrimer/probe sequenceGene targetAmplicon sizeGene position (nt)Ref
ureC-FM60398.15′-TGAGCGAATGCATGCGATT-3′ureC62 bp1447–1466This study
ureC-R5′-AATGATATGCCCGCTTTGCT-3’1489–1509
FAM-ureC-MGBEQ5′-ACAAAGCCAATTTTGGAGG-3’1467–1485
cagA-FX70039.15′-TCAAGAACCAGTTCCCCATGTC-3’cagA81 bp687–709This study
cagA-R5′-TCTCTAGCTTCAGGCGGTAAGC-3’746–768
HEX-cagA-MGBEQ5′-ACCAGATATAGCCACTACC-3’710–730