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Fig. 5 | BMC Microbiology

Fig. 5

From: New perspectives on butyrate assimilation in Rhodospirillum rubrum S1H under photoheterotrophic conditions

Fig. 5

Kinetic proteomic analysis of Rs. rubrum S1H cultivated under butyrate growth conditions. a Representation of the different sampling points used for kinetic proteomic analysis (T0, T1, T2, T3, and T4; n = 5). Growth in the presence of succinate (gray; n = 5) or butyrate (blue; n = 5) was monitored by measuring OD680nm. Red arrows represent bicarbonate supplementation (180 μmoles of NaHCO3, 6.5 ± 0.5 mM final). The color gradients above represent the expected concentration of butyrate (blue) and carbonate (red) in the culture medium, from most concentrated (color) to less concentrated (white). Green boxes indicate samples used for the first proteomic experiment comparing growth in medium supplemented with butyrate or succinate. b Principal component analysis of the proteomic data obtained from the kinetic proteomic experiment. Red, orange, yellow, light green and dark green dots represent the culture samples from the T0, T1, T2, T3 and T4 time points, respectively. Each axis represents a principal component (PC1 and PC2) with the percentage of the total variance it explains. The next two components (PC3 and PC4) explained 9.0 and 6.5% of the total variance, respectively

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