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Table 2 Primers for real-time PCR detection of tet(X3) and tet(X4) genes

From: A TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples

Primer

Sequence (5′-3′)

Gene

Product length

Accession No.

Reference

tet(X3)-qF

CAGGACAGAAACAGCGTTGC

tet(X3)

179 bp

MK134375.1

This study

tet(X3)-qR

GCAGCATCGCCAATCATTGT

tet(X4)-qF

TTGGGACGAACGCTACAAAG

tet(X4)

181 bp

MK134376.1

This study

tet(X4)-qR

CATCAACCCGCTGTTTACGC