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Fig. 7 | BMC Microbiology

Fig. 7

From: Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Fig. 7

Biofilm clearence ability of phages. Biofilms of A. hydrophila NJ-35 formed in 96-well plates for 24 h were treated with phage N21, W3 or G65 at concentrations of 108 PFU/mL, and biofilms of A. hydrophila XY-16 formed were treated with phage Y71 of Y81. Biofilms treated with SM buffer served as control. The biofilm left was stained with 1% crystal violet and measured at OD595 (bar chart). The percentage of biofilm left was calculated by dividing OD595 of phages treated wells and the OD595 of controls without phage treatment multiplied by 100. The percentage was subtracted from 100% in order to get the percentage of biofilm clearance (line chart). Data are shown as the mean ± SD from the triplicate experiments. ***P < 0.001, **P < 0.01 or *P < 0.05 indicates a significant difference between this group and the control with no phage treatment

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