Fig. 1
Inhibition of L. monocytogenes culture by morin. a Chemical structure of morin. b Growth kinetics curve of L95 cells treated with different concentrations of morin. L95 cells were incubated in TSB in the presence of various dosages of morin, and the growth of each sample was monitored every 30 min by measuring the value of OD600 nm at each indicated time point. c Suppression of the hemolytic activity of L95 culture supernatants by morin. L95 cells were co-cultured with morin, and the co-cultured supernatants were harvested by centrifugation. The hemolytic activity was assessed by a hemolysis assay. The hemolysis percent of each co-cultured supernatant was compared with that of the positive control group (treatment with Triton X-100). d The expression of LLO in L. monocytogenes cultures treated with different concentrations of morin was assessed by Western blotting using a specific antibody against LLO. Three independent trials were performed for each index. * indicates P < 0.05, and ** indicates P < 0.01. (Student’s t-test)