Fig. 4

Expression of the gvp gene cluster of Anabaena sp. PCC 7120 in E. coli. a Photographs of E. coli cells transformed with recombinant gvp plasmids and control vectors, respectively. b Negative-staining electron microscopy images of the putative gas vesicles purified from E. coli cells expressing the gvp genes (right) and the control vectors (left). c Western blot of the purified gas vesicles. The probe is anti-His antibody. The prestained protein standards are displayed in the lane marked Marker and their molecular masses are indicated in kDa. The lanes marked control and GV+ indicate the control and the experimental group, respectively