Fig. 4

Growth kinetics of M. smegmatis expressing LipF in the presence of RIF and STR. Squares represent LipF-expressing M. smegmatis, circles represent M. smegmatis-pMV261 control, and triangles represent wild-type M. smegmatis mc²155 strain. a Light gray lines correspond to bacterial growth in medium with 3.12 μg/mL RIF; black lines correspond to growth in medium with 1.6 μg/mL RIF; and dark gray lines correspond to growth in medium with 0.8 μg/mL RIF. b Light gray lines correspond to bacterial growth in medium with 0.25 μg/mL; STR black lines correspond to growth in medium with 0.12 μg/mL STR; and dark gray lines correspond to growth in medium with 0.06 μg/mL STR. Growth kinetics were determined in 7H9 medium supplemented with 10% ADC, 20 μg/mL kanamycin and RIF or STR at the indicated concentrations. Growth kinetics of M. smegmatis expressing LipF and its control (M. smegmatis-pMV261) are shown in the upper left of each drug. Solid lines represent 7H9 medium supplemented with 10% ADC and 20 μg/mL kanamycin (selection antibiotic) and discontinuous lines represent medium without kanamycin. Assays were performed in duplicate. Errors bars show standard erros, *, p ≤ 0.05 and ** p ≤ 0.01