Skip to main content

Table 1 List of inclusion and exclusion criteria for subjects enrollment in the study

From: Defining the oral microbiome by whole-genome sequencing and resistome analysis: the complexity of the healthy picture

Inclusion Exclusion
• Male and female subjects
• Age 18-30 years
• Good general health: free of systemic diseases such as diabetes, HIV infection or genetic disorder, ongoing malignant disease of any type that could interfere with the evaluation of the study objectives
• Good oral health: free of oral pathologies such as leukoplakia, erythroplakia, oral lichen planus (OLP)
• Availability for the 6-month duration of the study for an assigned subject
• Signed Informed Consent Form
• Pregnant or lactating women
• More than 8 missing teeth (with missing teeth, accounted for by third molar extractions, teeth extracted for orthodontic purposes, teeth extracted because of trauma, or congenitally missing teeth)
• Presence of orthodontic appliances
• Chronic dry mouth (clinically assessed)
• Significant halitosis (clinically assessed)
• Periodontitis
• Untreated carious lesions or oral abscesses
• Current or past (within 3 months before enrolment) assumption of medications that may influence oral microbiome (corticosteroids, calcium channel blockers, systemic antibiotics).
• Non-surgical and/or surgical mechanical/manual periodontal debridement within 3 months before enrolment.
• Heart diseases or blood pressure alteration that requires a medication
• Renal, hepatic, gastrointestinal disease that requires a medication
• Diabetes
• Presence of any sexually transmitted disease (STD), HIV, HCV infection
• Genetic disorders potentially interfering with the evaluation of the study objectives
• Chronic obstructive pulmonary disease and asthma
• Neoplastic lesions or paraneoplastic syndrome; tumors or significant pathology of the soft or hard tissues of the oral cavity (such as LPO, erythroplakia, leukoplakia, candidiasis)
• Current radiotherapy or chemotherapy