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Table 3 Bacterial identification results for isolates obtained from hot water systems and commercial compost, showing nearest matches based on BLAST alignment of complexity-reduced genotyping fragments. Average BLASTn percentage identity, nucleotide sequence distance values to best matches and genome coverage percentage are shown

From: Discovery of thermophilic Bacillales using reduced-representation genotyping for identification

Source

Sample name

Closest match

Average % identity

Nucleotide sequence distance (NSD)

Genome coverage (%)

Domestic hot water systems

 

DHWa

Geobacillus sp. 8

99.27

0.00446

2.28

 

DHWb

Geobacillus sp. 8

98.84

0.00719

2.12

 

DSP1a

Geobacillus lituanicus strain N-3

98.18

0.01177

1.80

 

DPS1b

Geobacillus lituanicus strain N-3

98.11

0.01174

1.80

 

DSP2a

Geobacillus lituanicus strain N-3

98.58

0.00838

3.25

 

DSP2b

Geobacillus lituanicus strain N-3

98.58

0.00855

3.23

 

DSP3

Geobacillus lituanicus strain N-3

98.61

0.00837

3.18

 

DSP4a

Geobacillus lituanicus strain N-3

98.55

0.00871

3.29

 

DSP4b

Geobacillus lituanicus strain N-3

98.60

0.00849

3.27

 

DSP5a

Geobacillus lituanicus strain N-3

98.29

0.01041

4.32

 

DSP5b

Geobacillus lituanicus strain N-3

98.33

0.01023

4.29

 

DPS5c

Geobacillus lituanicus strain N-3

98.35

0.01006

4.27

 

DSP6a

Geobacillus lituanicus strain N-3

98.51

0.00876

3.29

 

DSP6b

Geobacillus sp. MAS1 T260

98.37

0.00982

2.06

 

DSP6c

Geobacillus sp. MAS1 T260

98.35

0.00993

2.08

 

HTR

Geobacillus sp. MAS1 T260

98.29

0.01025

2.02

Commercial compost

 

DMW1

Geobacillus thermoleovorans strain ID-1

99.66

0.00189

4.54

 

CBSPa

Geobacillus thermodenitrificans strain G11MC16

99.39

0.00345

2.03

 

CBSPb

Geobacillus thermodenitrificans strain G11MC16

99.84

0.00099

2.10

 

CBSPc

Geobacillus thermodenitrificans strain G11MC16

99.83

0.00105

2.19

 

MFBBa

Geobacillus galactosidasius strain DSM 18751

98.97

0.00648

1.14

 

MFBBb

Geobacillus thermodenitrificans strain T12

99.59

0.00248

1.63

 

MFBBc

Geobacillus thermodenitrificans strain JSC_T9a

99.66

0.00212

1.10

 

MFBBd

Geobacillus galactosidasius strain DSM 18751

98.65

0.00804

1.14

 

MMBAa

Geobacillus galactosidasius strain DSM 18751

99.00

0.00671

1.28

 

MMBAb

Geobacillus galactosidasius strain DSM 18751

98.92

0.00674

1.12

 

MPCB

Geobacillus thermodenitrificans strain T12

99.58

0.00254

1.59

 

MPCC

Geobacillus thermodenitrificans strain G11MC16

99.56

0.00267

1.73

 

NFOSA1

Geobacillus thermodenitrificans strain G11MC16

99.83

0.00106

2.11

 

NFOSA2

Geobacillus sp. 8

98.69

0.00809

1.85

 

NFOSA3

Geobacillus galactosidasius strain DSM 18751

98.85

0.00725

1.14