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Table 2 Microbial growth for water and mud samples from the Great Artesian Basin. Incubation temperature was 62.5 °C. Culture media: LB broth agar (pH 5, pH 6.8, pH 8) and PBT pH 6.0

From: Discovery of thermophilic Bacillales using reduced-representation genotyping for identification

     

LB Broth

 

PBT

No. Bacterial isolates

Location

Source

Temperature (°C)

pH

Sample name

pH 5.0

pH 6.8

pH 8.0

pH 6.0

Birdsville

water

98 °Ca

nd

Birdsville Bore

nd

(+)

(+)

(−)

2

 

bore drain

98 °Ca

nd

Birdsville mud

nd

(+)(A), (+)(B)

(+)

(+)(A), (+)(B), (+)(C)

6

Clifton hills

water

80 °C

8.0

CHfil

(−)

(−)

(+)(A)

(−)

1

 

bore drain

80 °C

nd

CHMUD

(+)

(++)

(++)(A), (++)(B)

(++)

4

Mount Gason

water

80 °C

8.0

MtGfil

nd

(+)(A), (+)(B)

(+)(A)

(+)(A)

4

 

bore drain

80 °C

nd

Mt.GODS / Mt. GMUD

(+)(A), (+)(B)

(+)(A), (+)(B), (+)(C), (+)(D), (+)(E), (+)(F), (+)(G)

(+)(A), (+)(B), (+)(C),

(+)

13

Mirra Mita

water

79 °C

8.0

MMfil

nd

(+)

(−)

(−)

1

 

bore drain

79 °C

nd

MMMUD1

(−)

(+)

(−)

(+)

2

 

bore drain

68 °C

nd

MMMUD2

(++)

(++)

(−)

(−)

2

 

bore drain

74 °C

nd

MMMUD3

(−)

(++)

(+)(A)

(+)

3

 

bore drain

62 °C

nd

MMMUD4

(−)

(+)(A), (+)(B)

(+)(A), (+)(B)

(+)

5

 

bore drain

66 °C

nd

MMMUD5

(−)

(+)(A), (+)(B)

(−)

(−)

2

 

bore drain

50 °C

nd

MMMUD6

(−)

(+)(A), (+)(B)

(−)

(−)

2

 

bore drain

39 °C

nd

MMMUD7

(−)

(++)

(−)

(−)

1

 

bore drain

74 °C

nd

MMMUD8

(+)

(−)

(−)

(−)

1

Mungerannie station

water

78 °C

7.2

Mgnhotfil / MgnCCG

(+)

(+)

(+)

(−)

3

 

bore drain

60 °C

nd

MCWH

(−)

(+)(A), (+)(B)

(−)

(−)

2

Mulka

soil

38 °C

nd

MR

(−)

(+)(A)

(+)(A)

(−)

2

Kopperamanna

water

60 °C

8.5

Efil

(−)

(+)(A), (+)(B)

(−)

(++)

3

 

soil

38 °C

nd

ECO3

(−)

(−)

(−)

(++)

1

Etadunna station

water

77.9 °C

8.5

Kanufil

nd

(+)

(−)

(−)

1

Dulkaninna

water

47.8 °C

8.5

Dulfil

nd

(+)(+)

(−)

(−)

1

Clayton station

water

34 °C

8.5

Clfil

nd

(+)(A), (+)(B)

(−)

(−)

2

 

bore drain

34 °C

nd

CLB

(−)

(+)

(−)

(−)

1

Lake Harry

water

46 °C

8.5

LHfil

nd

(−)

(−)

(−)

 
 

bore drain

46 °C

nd

LHMUD

(−)

(+)(A), (+)(B)

(−)

(+)

3

Total bacterial isolates

        

68

  1. (a) Based on published temperature (Habermehl and Pestov 2002 [14])
  2. nd = not determined; (−) = no growth was observed; (+) = growth was observed; (++) = strong growth was observed; (A), (B), (C) name assigned if more than one microorganism was observed