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Fig. 1 | BMC Microbiology

Fig. 1

From: Discovery of thermophilic Bacillales using reduced-representation genotyping for identification

Fig. 1

Extract of report generated by bioinformatics pipeline Currito3.1 DNA Fragment Analyser [32] for sample MMMud_3_LB_ph8. This image shows the first candidate closest match to the sample. The circular graph plotted in Circos [33] shows the BLAST alignment position against the reference genome. The outer black circle represents the candidate reference genome with size indicated in megabases (Mb); the middle blue circle shows aligned sequenced fragments obtained by complexity-reduced genotyping and the Inner green / red circle shows the percentage identity of the alignments, in which values below 95% are red and values equal or above 95% are green. The bar plots show the sequences obtained with and without BLAST alignments against the best reference, in which the X axis shows the sequences classification of sequences with and without hits to a reference or plasmid and the Y axis shows the total number of sequences. The histogram shows the percentage identity of BLAST alignments against the candidate reference, where the X axis shows the BLAST alignment percentage identity highest to lowest and the Y axis has the number of aligned sequences

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