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Table 1 E. coli strains and plasmids used in this study

From: A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate

Strain or plasmidDescriptionSource
Strains
E. coli REL606E. coli str. B F, tsx-467(Am), araA230, lon, rpsL227(strR), hsdR, [mal+](LamS)Richard E. Lenski
E. coli BL21(DE3)E. coli str. B FompT gal dcm lon hsdSB(rBmB) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12S)F. William Studier
Plasmids
 pHCE-IIBExpression vector carrying the ampicillin resistance gene and a high-level constitutive expression promoter (HCE)[31]
 pHCE-IIB-HpaB(R379)pHCE-IIB carrying hpaB with arginine at the 379th position [equivalent to hpaB of E. coli BL21(DE3)]This study
 pHCE-IIB-HpaB(C379)pHCE-IIB carrying hpaB with cysteine at the 379th position (equivalent to hpaB of E. coli REL606)This study
 pHCE-IIB-HpaB(G379)pHCE-IIB carrying hpaB with glycine at the 379th position (R379G substitution)This study
 pHCE-IIB-HpaB(S379)pHCE-IIB carrying hpaB with serine at the 379th position (R379S substitution)This study