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Fig. 2 | BMC Microbiology

Fig. 2

From: A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate

Fig. 2

Growth curves of hpaB-expressing REL606 in defined medium with 3HPA as sole carbon source. E. coli REL606 cells harboring expression constructs BL21(DE3)-derived hpaB [pHCE-IIB-HpaB(R379), ] and REL606-derived hpaB [pHCE-IIB-HpaB(C379), ] are shown. REL606 harboring the control plasmid (pHCE-IIB, ) was used as the control. The culture medium comprised defined medium supplemented with 3 g/L (20 mM) 3HPA. Only REL606 harboring pHCE-IIB-HpaB(R379) grew on 3HPA, and concentrations of 3HPA in the culture supernatant (denoted as white squares with a dashed line) and HPC (red squares with a dashed line) were quantified. Error bars denote the standard deviation of mean from three independent cultures

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