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Fig. 6 | BMC Microbiology

Fig. 6

From: Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry

Fig. 6

DNA-binding activity of LiaR investigated by EMSA. a LiaR was incubated with PliaI at different concentrations. Samples were incubated for 30 min at room temperature. They were resolved in a 9% native-PAGE. The gels were exposed to a phosphor screen and imaged by a Typhoon Trio+ imager. b LiaR treated with acetyl phosphate, was incubated with PliaI and analyzed as described above. c LiaRC was incubated with PliaI and analyzed as described above

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