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Fig. 5 | BMC Microbiology

Fig. 5

From: Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry

Fig. 5

Oligomerization studies on LiaR. The oligomerization state of LiaR was analyzed by a 10% native-PAGE. Samples with or without acetyl phosphate (AcP) were incubated for 1 h in PB, at room temperature, and were resolved by gel electrophoresis. VraR was used as a control of the effect of phosphorylation in the oligomerization state of the protein. LiaS at 10 μM was added to investigate its phosphatase activity and its effect on the oligomerization of LiaR

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BMC Microbiology

ISSN: 1471-2180

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