Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry

Fig. 3

Phosphotransfer between LiaS and LiaR. Phosphorylated LiaS at 4 μM was incubated with 20 μM LiaR in PB at room temperature. The reaction was quenched at various time intervals and samples were analyzed by 15% SDS-PAGE. Gels were exposed to a phosphor screen (top panel) and imaged using a Typhoon Trio+ Imager (GE Healthcare), followed by coomassie staining (bottom panel)

Back to article page