Fig. 2

The autokinase activity of LiaS. a LiaS at 5 μM was incubated with 25 μM [γ-³²P] ATP in PB at 25 °C. The reaction was quenched at different time intervals and samples were analyzed by a 12.5% SDS-PAGE. The SDS-PAGE was scanned with a phosphor screen to quantify the incorporation of [γ-³²P] to LiaS (top pannel). The bottom panel is a coomassie stain of the SDS-PAGE to ensure equal loading of the samples. b The observed rate constants were plotted against ATP concentration to determine the k^app and K_S