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Fig. 7 | BMC Microbiology

Fig. 7

From: Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Fig. 7

Associations between reproducibility and a participant age at specimen collection, b 16S rRNA gene copy numbers, and c read counts. Reproducibility is measured by coefficient of determination (R2) values, calculated by comparing proportions of each OTU present between technical repeats. Horizontal blue bars highlight R2 values > 0.90. Different shades of vertical blue bars represent a< 7, < 14, < 30, < 60 days; b< 100, < 500, < 1000 copies/μl; and c< 2000, < 4000, < 6000, < 8000 and < 10,000 reads; respectively. For b and c, each set of technical repeats had two 16S rRNA gene copy number/read count measures shown as two points connected by a horizontal line on the X-axis

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