Fig. 6

Bacterial composition in no template controls (Primestore, n = 35) and low biomass technical repeats (n = 209). a Dendogram representing unsupervised hierarchical clustering distances are based on Bray Curtis dissimilarity indices calculated at OTU-level. The dendogram is colour-coded based on specimen type (Primestore: darkturquoise; technical repeats: deeppink). b Differences between Primestore and technical repeats are shown at genus-level, with colour-codes representing phylum-level classification (Shades of blue: Proteobacteria, shades of red: Firmicutes). Genera with proportions < 1% in each of the specimens are grouped together as “Other” and shown in grey. c Most abundant genera within each the specimens, specimen type, participant age at specimen collection (in days) and 16S rRNA gene copy numbers (copies/μl) are summarised at the bottom of the Fig. A-N-P-R: Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium