Fig. 4From: Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimensParticipant age at specimen collection, read counts and alpha diversity relative to specimen biomass for no template controls (Primestore, n = 35) and technical repeats (n = 209). a Scatter plot of participant age at specimen collection b read counts following bioinformatic processes and c Shannon diversity indices (alpha diversity) at OTU-level in relation to specimen biomass (16S rRNA gene copies/μl) plotted on loge scale. Vertical pink shaded area highlights 16S rRNA gene copies/μl < 500Back to article page