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Fig. 4 | BMC Microbiology

Fig. 4

From: Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Fig. 4

Participant age at specimen collection, read counts and alpha diversity relative to specimen biomass for no template controls (Primestore, n = 35) and technical repeats (n = 209). a Scatter plot of participant age at specimen collection b read counts following bioinformatic processes and c Shannon diversity indices (alpha diversity) at OTU-level in relation to specimen biomass (16S rRNA gene copies/μl) plotted on loge scale. Vertical pink shaded area highlights 16S rRNA gene copies/μl < 500

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