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Fig. 1 | BMC Microbiology

Fig. 1

From: Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Fig. 1

Representation of a expected and b actual sequencing profiles from no template controls, low biomass and high biomass specimens following 16S rRNA gene sequencing. a Expected 16S rRNA gene sequencing profiles from i) no template controls (NTCs), ii) low biomass and iii) high biomass biological specimens which corresponds with their endogenous bacterial composition. b Actual 16S rRNA gene sequencing profiles generated from i) NTCs may comprise of reagent and laboratory contaminants as well as exogenous sequences from low and high biomass specimens (well-to-well contamination); ii) low biomass biological specimen sequencing profiles may be overrepresented by exogenous profiles from both NTCs (reagent and laboratory contaminants) and high biomass specimens (well-to-well contamination); whilst iii) high biomass sequencing profiles are expected to be least affected by reagent and laboratory contaminants present in NTCs and cross-contamination from low biomass specimens

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