Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Lima, Magna Coroa; de Barros, Mariana; Scatamburlo, Thalita Moreira; Polveiro, Richard Costa; de Castro, Laís Karolyne; Guimarães, Samuel Henrique Sales; da Costa, Sanely Lourenço; da Costa, Mateus Matiuzzi; Moreira, Maria Aparecida Scatamburlo

doi:10.1186/s12866-020-01793-9

Table 3 Primers used in the detection of Staphylococcus aureus resistance, multidrug efflux system and virulence genes

From: Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Category	Gene	Primer	Sequence	Product (bp)	Reference
Resistance	mecA	mecA - f	CCTAGTAAAGCTCCGGAA	314	[59]
	mecA	mecA - r	CTAGTCCATTCGGTCCA	314	[59]
	Aac(6′)/aph(2′)	Aac(6′)/aph(2′) - f	GAAGTACGCAGAAGAGA	491	[59]
	Aac(6′)/aph(2′)	Aac(6′)/aph(2′) - r	ACATGGCAAGCTCTAGGA	491	[59]
	aph(3′)-IIIa	aph(3′)-IIIa - f	AAATACCGCTGCGTA	242	[59]
	aph(3′)-IIIa	aph(3′)-IIIa - r	CATACTCTTCCGAGCAA′	242	[59]
	ant(4′)-Ia	ant(4′)-Ia - f	AATCGGTAGAAGCCCAA	135	[59]
	ant(4′)-Ia	ant(4′)-Ia - r	GCACCTGCCATTGCTA	135	[59]
	tet(M)	tet(M) - f	AGTGGAGCGATTACAGAA	360	[60]
	tet(M)	tet(M) - r	CATATGTCCTGGCGTGCTTA	360	[60]
	tet(K)	tet(K) - f	GTAGCGACAATAGGTAATAGT	158	[60]
	tet(K)	tet(K) - r	GTAGTGACAATAAACCTCCTA	158	[60]
	blaZ	blaZ - f	ACTTCAACACCTGCTGCTTTC	173	[60]
	blaZ	blaZ - r	TGACCACTTTTATCAGCAACC	173	[60]
	ermA	ermA - f	TATCTTATCGTTGAGAAGGGATT	139	[61]
	ermA	ermA - r	CTACACTTGGCTTAGGATGAAA	139	[61]
	ermB	ermB - f	CTATCTGATTGTTGAAGAAGGATT	142	[61]
	ermB	ermB - r	GTTTACTCTTGGTTTAGGATGAAA	142	[61]
	ermC	ermC - f	CTTGTTGATCACGATAATTTCC	299	[61]
	ermC	ermC - r	ATCTTTTAGCAAACCCGTATTC	299	[61]
Multidrug Efflux Pump	tet38	tet38 - f	TTCAGTTTGGTTATAGACAA	400	[61]
	tet38	tet38 - r	CGTAGAAATAAATCCACCTG	400	[61]
	norA	norA - f	TGCAATTTCATATGATCAATCCC	150	[29]
	norA	norA - r	AGATTGCAATTCATGCTAAATAT	150	[29]
	norB	norB - f	ATAAGGTAAGATAACTAGCA	150	[29]
	norB	norB - r	ATCTCTATTTGCCTCCCTATA	150	[29]
	norC	norC - f	AAATGGTTCTAAGCGACCAA	200	[29]
	norC	norC - r	ATAAATACCTGAAGCAACGC	200	[29]
	LmrS	LmrS - f	TAAAGTTGAATTAACAAC	180	[30]
	LmrS	LmrS - r	GCGGATCCTTAAAATTTC	180	[30]
	mgrA	mgrA - f	CGAATTCATTCATGATTT	200	[61]
	mgrA	mgrA - r	AAAGTTGATTGTTTATTAA	200	[61]
	msrA	msrA - f	TCCAATCATAGCACAAAATC	163	[61]
	msrA	msrA - r	AATTCCCTCTATTTGGTGGT	163	[61]
Virulence	hla	hla - f	CTGATTACTATCCAAGAAATTCGATTG	209	[62]
	hla	hla - r	CTTTCCAGCCTACTTTTTTATCAGT	209	[62]
	fnbA	fnbA - f	GTGAAGTTTTAGAAGGTGGAAAGAITAG	643	[63]
	fnbA	fnbA - r	GCTCTTGTAAGACCATTTTTCTTCAC	643	[63]
	fnbB	fnbB - f	GTAACAGCTAATGGTCGAATTGATACT	524	[63]
	fnbB	fnbB - r	CAAGTTCGATAGGAGTACTATGTTC	524	[63]
	eta	eta - f	ACTGTAGGAGCTAGTGCATTTGT	190	[64]
	eta	eta - r	TGGATACTTTTGTCTATCTTTTTCATCAAC	190	[64]
	etb	etb - f	CAGATAAAGAGCTTTATACACACATTAC	621	[64]
	etb	etb - r	AGTGAACTTATCTTTCTATTGAAAAACACTC	621	[64]
	lukDE	lukDE - f	TGAAAAAGGTTCAAAGTTGATACGAG	269	[64]
	lukDE	lukDE - r	TGTATTCGATAGCAAAAGCAGTGCA	269	[64]
	tst	tst- f	TTCACTATTTGTAAAAGTGTCAGACCCACT	180	[64]
	tst	tst - r	TACTAATGAATTTTTTTATCGTAAGCCCTT	180	[64]
	sea	sea - f	ACGATCAATTTTTACAG	544	[65]
	sea	sea - r	TGCATGTTTTCAGAGTTAATC	544	[65]
	seb	seb - f	GAATGATATTAATTCGCATC	416	[65]
	seb	seb - r	TCTTTGTCGTAAGATAAACTTC	416	[65]
	sec	sec - f	GACATAAAAGCTAGGAATTT	257	[65]
	sec	sec - r	AAATCGGATTAACATTATCCA	257	[65]
	sed	sed - f	CTAGTTTGGTAATATCTCCT	317	[65]
	sed	sed - r	TAATGCTATATCTTATAGGG	317	[65]
	see	see - f	TAGATAAAGTTAAAACAAG	170	[65]
	see	see - r	TAACTTACCGTGGACCCTTC	170	[65]
	seg	seg - f	GTTAGAGGAGGTTTTATG	198	[65]
	seg	seg - r	TTCCTTCAACAGGTGGAGA	198	[65]
	seh	seh - f	CAACTGCTGATTTAGCTCAG	173	[65]
	seh	seh - r	CCCAAACATTAGCACCA	173	[65]
	sei	sei - f	GGCCACTTTATCAGGACA	328	[65]
	sei	sei - r	AACTTACAGGCAGTCCA	328	[65]
	sej	sej - f	GTTCTGGTGGTAAACCA	131	[65]
	sej	sej - r	GCGGAACAACAGTTCTGA	131	[65]

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ISSN: 1471-2180

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