Skip to main content

Table 4 Primers used in this study

From: Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine

Primer

Description or purpose

Sequence

Pox_F_NdeI

Cloning of Δ34pox into pFLAG-CTC

AGATATCATATGGCAAAAATTAAGGGCGCAAAC

Pox_R_Δ34_XhoI

Cloning of Δ34pox into pFLAG-CTC, includes His6-tag and stop codon

AATTCCCTCGAGTTAGTGATGGTGATGGTGATGACTTCCTGCACCTGAAGCCGCGTCAATTTC

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us