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Fig. 2 | BMC Microbiology

Fig. 2

From: Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine

Fig. 2

Oligomeric state of L. delbrueckii Pox. a. Size exclusion chromatography of L. delbrueckii Pox. 840 μg of Pox was combined with 50 mM pyruvate, 300uM TPP, and 15uM FAD and applied to the column. The absorbance at 280 nm is shown along with the elution position of known protein standards indicated by arrows. The calculated weight of the L. delbrueckii Pox is indicated based on the standard curve. 1 mL fractions were collected beginning at 10 mL elution volume to the end of the elution. b. Standard curve of gel filtration elution volumes for known protein standards. c. Coomassie stain of indicated fractions from gel filtration after SDS-PAGE under non-reducing conditions. d. Coomassie stain of purified L. delbrueckii Pox after SDS-PAGE under reducing (5% beta-mercaptoethanol) and non-reducing conditions

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