In vitro conjugation kinetics of AmpC, broad spectrum and extended-spectrum beta-lactamase-producing Escherichia coli donors and various Enterobacteriaceae recipients

Table 2 Conjugation frequencies of selected donor- and recipient strains [log₁₀transconjugants/donor]

Donor	*E. coli* ESBL10682				*E. coli* ESBL10689		*E. coli* ESBL10708		*E. coli* ESBL10717
Recipient	*E. coli* IMT 20751/402	*E. coli* IMT11716	*S. enterica* serovar Typhimurium L1219-R32	*Serratia marcescens* subsp. *marcescens* DSM 31022	*E. coli* IMT 20751/402	*Serratia marcescens* subsp. *marcescens* DSM 31022	*Enterobacter cloacae* DSM 30060	*S. enterica* serovar Typhimurium L1219-R32	*E. coli* IMT 20751/402	*E. coli* IMT 11716	*Enterobacter cloacae* DSM 30060
0 h	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
2 h	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
4 h	−7.00	NT	−4.98	−5.74	−7.26	NT	NT	NT	NT	NT	NT
6 h	−6.62	NT	−6.27	−7.05	−7.30	NT	NT	NT	NT	NT	NT
8 h	−6.70	NT	−5.91	−6.71	−8.48	− 6.91	NT	NT	NT	NT	NT
22 h	−6.78	−6.52	−5.92	−7.26	− 6.45	−6.98	− 5.86	−5.70	−6.80	− 6.25	−6.49

NT no transconjugants; experiments were performed in duplicates and the results were reported as their average (coefficients of variation < 10.6%)

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