Table 3 Primer nucleotide sequences used in qRT-PCR assay
From: Seasonal variation in gut microbiota composition: cross-sectional evidence from Ukrainian population
Phylum | Primer nucleotide sequence | |
|---|---|---|
Forward | Reverse | |
Bacteroidetes | 798cfbF AAACTCAAAKGAATTGACGG | cfb967R GGTAAGGTTCCTCGCGCTAT |
Firmicutes | 928F-firm TGAAACTYAAGGAATTGACG | 1040FirmR ACCATGCACCACCTGTC |
Actinobacteria | Act920F3 TACGGCCGCAAGGCTA | Act1200R TCRTCCCCACCTTCCTCCG |
16S rRNA gene | 926F AAACTCAAAKGAATTGACGG | 1062R CTCACRRCACGAGCTGAC |