Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate

BMC Microbiology

Table 4 Slope, R², and efficiency of Lactobacillus reference strain in the PCR assay

Species	Slope	R²	Efficiency (%)
L. gasseri	−3.214	0.999	104.701
L. rhamnosus	− 3.362	0.998	98.35
L. brevis	− 3.444	1	95.158
L. sakei	− 3.212	1	104.797
L. johnsonii	−3.214	0.999	104.701
L. jensenii	−3.328	0.996	99.764
L. fermentum	−3.56	0.995	90.955
L. plantarum	−3.221	0.995	104.396
L. paracasei	−3.305	0.98	100.694
L. paraplantarum	−3.256	0.998	102.822
L. casei	−3.284	0.999	101.612
L. curvatus	−3.485	0.999	93.617
L. acidophilus	−3.506	1	92.845
L. salivarius	−3.564	1	90.809
L. reuteri	−3.342	0.999	99.161
L. coryniformis	−3.217	0.989	104.578
L. farciminis	−3.386	0.991	97.39
L. zymae	−3.5	0.997	93.073
L. pentosus	−3.292	0.999	101.251
L. crustorum	−3.438	0.999	95.366
L. mucosae	−3.478	0.986	93.886
L. buchneri	−3.411	0.993	96.424
L. helveticus	−3.230	0.998	103.98
L. amylovorus	−3.582	0.993	90.167
L. heilongjiangensis	−3.462	1	94.458
L. parabuchneri	−3.207	1	105.049
L. acidipiscis	−3.528	0.984	92.075
L. sanfranciscensis	−3.229	0.999	104.034
L. ruminis	−3.295	1	101.153
L. agilis	−3.508	1	92.795
L. delbrueckii	−3.31	0.999	100.479
L. amylophilus	−3.481	0.984	93.768
L. kunkeei	−3.571	0.998	90.568
L. acetotolerans	−3.209	1	104.92
L. lindneri	−3.559	0.982	90.972
L. gallinarum	−3.346	0.999	98.989
L. amylolyticus	−3.552	0.996	91.209

ISSN: 1471-2180