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Table 1 Information of primer pairs designed for this study

From: Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate

SpeciesTarget genePrimer nameSequence (5′–3′)Product
size (bp)
Primer
conc.b (μM)
IPCa16S–23S regionIPC-FCAA CGC GAA GAA CCT TAC CAG1110.4
 IPC-RCCA ACA TCT CAA CGA CAC GAG C  
L. gasseri16S–23S regionGasseri-FTCA AGA GCT GTT AAG GCT GT1750.04
 Gasseri-RCTA TCG CTT CAA GTG CTT TC  
L. rhamnosus16S–23S regionRhamnosus-FGCC GAT CGT TGA CGT TAG TTG G1370.04
 Rhamnosus-RCAG CGG TTA TGC GAT GCG AAT  
L. brevis16S–23S regionBrevis-FGGG CAA CGA AGC AAG ATC GC2600.08
 Brevis-RTTC CAA TCG TGT GCA CAC CA  
L. sakei16S–23S regionSakei-FTCG AAC GCA CTC TCG TTT AG1820.08
 Sakei-RCGA AAC CAT CTT TCA ACC CT  
L. johnsonii16S–23S regionJohnsonii-FAGA GAG AAA CTC AAC TTG AAA TA1950.4
 Johnsonii-RCCT TCA TTA ACC TTA ACA GTT AA  
L. jensenii16S–23S regionJensenii-FAGT TCT TCG GAA TGG ACA TAG1480.4
 Jensenii-RGCC GCC TTT TAA ACT TCT T  
L. fermentumUnique geneFermentum-FGAC CAG CGC ACC AAG TGA TA1290.08
 Fermentum-RAGC GTA GCG TTC GTG GTA AT  
L. plantarumUnique genePlantarum-FGCT GGC AAT GCC ATC GTG CT1470.12
 Plantarum-RTCT CAA CGG TTG CTG TAT CG  
L. paracaseiUnique geneParacasei-FCAA TGC CGT GGT TGT TGG AA1060.4
 Paracasei-RGCC AAT CAC CGC ATT AAT CG  
L. paraplantarumUnique geneParaplantarum-FTTA TTC AAG CCG TCG GAG TG1280.4
 Paraplantarum-RTCG CTG GTG CTA ATG CAA TG  
L. caseiUnique geneCasei-FCCA CAA TCC TTG GCT GTT CT1150.4
 Casei-RGCT TGA GGC GAT TGT AAT CC  
L. curvatus16S–23S regionCurvatus-FACT CTC ATT GAA TTA GGA CGT T1320.4
 Curvatus-RCCC GTG TTG GTA CTA TTT AAT  
L. acidophilus16S–23S regionAcidophilus-FCCT TTC TAA GGA AGC GAA GGA T1290.4
 Acidophilus-RACG CTT GGT ATT CCA AAT CGC  
L. salivarius16S–23S regionSalivarius-FTAC ACC GAA TGC TTG CAT TCA1380.08
 Salivarius-RAGG ATC ATG CGA TCC TTA GAG A  
L. reuteri16S–23S regionReuteri-FGAT TGA CGA TGG ATC ACC AGT1610.2
 Reuteri-RCAT CCC AGA GTG ATA GCC AA  
L. coryniformis16S–23S regionCoryniformis-FCAA GTC GAA CGC ACT GAC G1650.4
 Coryniformis-RACA TTC AGG CCA TGT GGT CT  
L. farciminisUnique geneFarciminis-FACG AAT CCG GCA GTC AAG AA1520.08
 Farciminis-RAAG AAT CGC CAA GCT CTA GG  
L. zymae16S–23S regionZymae-FGCT AAA GCA AGC GCA CGA TT1320.08
 Zymae-RTCG GCA GTG TGA CAT GGA G  
L. pentosusUnique genePentosus-FGCG GTA TCG ATT CGA TTG GT1450.08
 Pentosus-RTGA TGT CAA TCG CCT CTT GG  
L. crustorum16S–23S regionCrustorum-FGGA ATA GCC CAA ACC AGA G1450.2
 Crustorum-RACT GAA TGG AGT GGG TCA GA  
L. mucosae16S–23S regionMucosae-FACG GAC TTG ACG TTG GTT TAC1560.4
 Mucosae-RGTG ATA GCC GAA ACC ACC TT  
L. buchneri16S–23S regionBuchneri-FCAA GTC GAA CGC GTC TCC AT1890.08
 Buchneri-RCCG AAG CCG TCT TTT AAA CC  
L. helveticusUnique geneHelveticus-FCTA CTT CGC AGG CGT TAA CT1320.08
 Helveticus-RGTA CTT GAT GCT CGC ATA CC  
L. amylovorusUnique geneAmylovorus-FCAA GCA CGA TTG GCA AGA TG1260.4
 Amylovorus-RATT GGA TTC CGC TTC TGT GG  
L. heilongjiangensis16S–23S regionHeilongjiangensis-FGCT TCA TGA ATC GGA TCT AA1330.4
 Heilongjiangensis-RTAA ACT ACG ATC ATG TGA AAG TA  
L. parabuchneriUnique geneParabuchneri-FAGC GTC GTG ATT CCT GAT AC1370.08
 Parabuchneri-RCGA CTC TCC GAT CGT TGT TA  
L. acidipiscisUnique geneAcidipiscis-FAGC GGT TCG ATG GCT TAT AC1250.08
 Acidipiscis-RTCC AAG TCC GAC ACC AGT CA  
L. sanfranciscensisUnique geneSanfranciscensis-FTGG AAC TGA TAC GCG GAT GT1300.08
 Sanfranciscensis-RGGC CAA TTC CTC CAA TAA CG  
L. ruminis16S–23S regionRuminis-FTTG CAT TCA CCG AAA GAA GC1290.4
 Ruminis-RCAT AAA CAT CAT GCG GTG TTC  
L. agilis16S–23S regionAgilis-FTCG TAG CTT GCT ACA CCG ATT G1370.4
 Agilis-RCAT AAT GAC CAT GCG ATC ATC A  
L. delbrueckii16S–23S regionDelbrueckii-FCAT GTG CAG ACA TGC TAT CCT T1920.4
 Delbrueckii-RCTC TGA AGT GCC ATG TCT CAG T  
L. amylophilus16S–23S regionAmylophilus-FCGA GTT CTG GTT AAG AGT AGC G1740.4
 Amylophilus-RCGC CAT CTT TCA AAC ATC TAT C  
L. kunkeei16S–23S regionKunkeei-FGAA CGA GCT CTC CCA AAT TGA1610.4
 Kunkeei-RGAA CCA TGC GGT TCC AAC TA  
L. acetotolerans16S–23S regionAcetotolerans-FGAT TAC CTT CGG GTA TGA AGT T1310.2
 Acetotolerans-RTCA TGT GAT CTC TCC TTT TAT CC  
L. lindneriUnique geneLindneri-FCGG CGT TCT CGA GGA CCA TA1700.4
 Lindneri-RCAT CCG GCG TCC TTC ATA GC  
L. gallinarumUnique geneGallinarum-FAAC TGG CGG TTA TCG TAG AC1180.2
 Gallinarum-RCAC AGC AGG AAC CAT TTT AG  
L. amylolyticus16S–23S regionAmylolyticus-FTTC GGT AGT GAC GTT TCG GA1340.2
 Amylolyticus-RTCA AGC AAG TGC CAT GCA G  
  1. aIPC, internal positive control
  2. bconc., concentration