Table 3 Evaluation of select drug candidates against B. henselae biofilm-recovered cells after drug exposure at different times
Drugs (5 μg/ml) | CFU per mL after drug exposure | ||
|---|---|---|---|
2 day | 4 day | 6 day | |
Drug free control | 1.3 ± 0.2 × 1010 | 1.8 ± 0.2 × 1010 | 2.6 ± 0.3 × 1010 |
Azithromycin | 4.5 ± 0.3 × 109 | 5.2 ± 0.3 × 109 | 9.3 ± 0.2 × 105 |
Cefuroxime | 5.6 ± 0.3 × 109 | 2.3 ± 0.2 × 109 | 1.7 ± 0.1 × 106 |
Ciprofloxacin | 3.2 ± 0.3 × 108 | 2.5 ± 0.3 × 108 | 5.1 ± 0.3 × 102 |
Daptomycin | 9.8 ± 0.2 × 109 | 3.4 ± 0.3 × 109 | 2.7 ± 0.2 × 106 |
Disulfiram | 6.1 ± 0.3 × 109 | 4.6 ± 0.3 × 109 | 3.8 ± 0.3 × 107 |
Doxycycline | 5.3 ± 0.3 × 109 | 3.8 ± 0.1 × 109 | 6.2 ± 0.3 × 105 |
Gentamicin | 6.2 ± 0.3 × 109 | 5.8 ± 0.2 × 109 | 8.1 ± 0.3 × 102 |
Methylene blue | 8.9 ± 0.4 × 109 | 6.8 ± 0.2 × 109 | 2.3 ± 0.2 × 102 |
Miconazole | 9.8 ± 0.3 × 109 | 2.2 ± 0.1 × 1010 | 1.6 ± 0.1 × 1010 |
Nitrofurantoin | 4.3 ± 0.1 × 109 | 3.6 ± 0.2 × 109 | 2.8 ± 0.2 × 109 |
Rifampin | 5.6 ± 0.2 × 109 | 4.8 ± 0.3 × 109 | 3.2 ± 0.2 × 102 |
SXT | 7.8 ± 0.3 × 109 | 6.4 ± 0.3 × 109 | 2.7 ± 0.2 × 109 |
Azithromycin+Ciprofloxacin | 4.1 ± 0.2 × 109 | 5.8 ± 0.3 × 106 | 0 |
Azithromycin+Methylene blue | 6.8 ± 0.2 × 109 | 5.2 ± 0.3 × 105 | 0 |
Rifampin+Ciprofloxacin | 7.6 ± 0.2 × 109 | 4.7 ± 0.2 × 109 | 0 |
Rifampin+Methylene blue | 5.9 ± 0.1 × 109 | 4.8 ± 0.2 × 109 | 0 |