Table 2 Amplicon sizes (base pairs) obtained from multiplex PCR amplification using yeast specific (Universal-UNI1 and UNI2) and corresponding species-specific primers for four different Candida spp.
Species | Primer | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
UNI 1 UNI 2 | GTCAAACTTGGTCATTTA TTCTTTTCCTCCGCTTATTG | ||
C. albicans | Calb | AGCTGCCGCCAGAGGTCTAA | 583/446 |
C. tropicalis | Ctro | GATTTGCTTAATTGCCCCAC | 583/507 |
C. krusei | Ckru | CTGGCCGAGCGAACTAGACT | 590/169 |
C. glabrata | Cgla | TTGTCTGAGCTCGGAGAGAG | 929/839 |